Table 2.

Diagnostic performance of the 18S TaqMan real-time PCR assay

Characteristic	Calculations	Value	95% CI
Sensitivity	(true positives)/(true positives + false negatives)	36/(36 + 0) × 100 = 100%	90.3–100.0%
Specificity	(true negatives)/(true negatives + false positives)	42/(42 + 0) × 100 = 100%	91.6–100.0%
Accuracy	(true positives + true negatives)/(true positives + true negatives + false negatives + false positives)	(36 + 42)/(36 + 42 + 0 + 0) × 100 = 100%	95.3–100.0%
Reproducibility	(results in agreement)/(total no. of results)	467/468 × 100 = 99.8%	98.8–100.0%

CI = confidence interval; PCR = polymerase chain reaction.