Figure 2.
Inhibition of exogenous HIV by ImmTAV-redirected CD8+ T-cells. Primary CD4+ T-cells from healthy HLA-A*0201-positive donors were infected with HIV-IIIB and cultured alone (5 × 104 cells/well), with autologous CD8+ T-cells only or with CD8+ T-cells plus HIV ImmTAVs, m121 and m134, or an irrelevant TCR-anti-CD3 scFV fusion (control TCR). (a, b). Frequencies of HIV-infected (p24 Ag+) CD4+ T-cells and (c, d) percent inhibition after 7 days' culture at CD8+/CD4+ cell ratios of 1:1 a, c and 1:10 b, d; ImmTAVs were tested at concentrations ranging from 10–8 – 10–11 mol/l. Data shown are mean ± SD and are representative of three experiments. Percent inhibition was calculated as described in Materials and Methods. (e) Saturation of CD8+ T-cells with ImmTAVs: CD8+ T-cells were incubated with ImmTAVs at concentrations indicated, before staining with an HLA-A*0201/SL9 dextramer and analysis by flow cytometry. Dextramer binding is shown in dot plots (top) at ImmTAV concentrations of 0, 10–8, and 10–9 mol/l and in histograms (bottom) at ImmTAV concentrations of 10–8 – 10–12 mol/l for each TCR. HLA, human histocompatibility leukocyte antigen; HIV, human immunodeficiency virus; TCR, T-cell receptor.