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. 2016 Jul 12;24(11):1913–1925. doi: 10.1038/mt.2016.114

Figure 3.

Figure 3

ImmTAV-mediated inhibition of autologous HIV spread in CD4+ T-cells from HIV-infected subjects. Purified CD4+ T-cells (1 × 105) from 10 HIV-positive HLA-A*0201+ subjects (8 ART-treated and 2 ART-naive, represented by symbols shown above) were stimulated with PHA for 72 hours to promote replication of endogenous HIV, then cultured with autologous CD8+ T-cells at CD8+/CD4+ cell ratios of 1:1 (a) and 1:10 (b), either alone or with ImmTAVs as indicated (ImmTAVs at 10–8 mol/l for the 1:1 ratio, 10–11 mol/l for the 1:10 ratio). The irrelevant TCR-anti-CD3 scFV fusion (control TCR) was tested at 10–9 mol/l and 10–12 mol/l for the 1:1 and 1:10 ratios respectively because of nonspecific cell death at higher concentrations. Reduction in Gag+ cells was determined on day 7 of coculture. Horizontal lines indicate mean values. HLA, human histocompatibility leukocyte antigen; HIV, human immunodeficiency virus; ImmTAV, immune-mobilising monoclonal T-cell receptors against virus; TCR, T-cell receptor.