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. 2016 Oct 4;24(11):1965–1973. doi: 10.1038/mt.2016.168

Figure 3.

Figure 3

Transduced LN stromal cells are differentially activated following administration of LV-MHCII or LV-MHCII-miR vectors. C57Bl/6 female mice were injected intravenously (IV) with 50 ng reverse transcriptase (RT) of LV-MHCII or LV-MHCII-miR, or PBS as control. After 3 days, LN were harvested and analyzed by FACS (pool of three mice per group per experiment) (a) Representative expression of CD86, CTLA-4, B7-H4 activation markers by transgene expressing cells in LN. Results representative of two (B7-H4) and three (CD86, CTLA-4) independent experiments. (b) Averaged percentage of live CD45− GFP-HY+ cells expressing the indicated markers and SD, from the experiments in a. The parent cell population is CD45− live cells. GFP, green fluorescent protein; LN, lymph node; LV, lentiviral vectors; PBS, phosphate buffered saline; SD, standard deviation.