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. 2016 Dec 13;11(12):e0166657. doi: 10.1371/journal.pone.0166657

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Fig 1 — (A) Cell diameters of stemRBCs (n = 10) derived from cord blood isolated CD34⁺ cells during differentiation from day 0 to day 18 in culture compared to human control RBCs and filtered cells on day 18. (B) Cell morphology of adult human blood (control) from cytospins for comparison with pre- (NF) and post-filter (F) day 18 stemRBCs. Scale bar represents 20 μm. (C) Total number of viable, non-adherent cells were determined and plotted as a mean ± SE of 4 cultures. (D) Frequency of cells expressing several erythroid markers as determined by flow cytometry over an 18 day culture period (mean ± SE) and (E) mean ± SE fluorescence intensities (MFIs) showing expression patterns of these markers during differentiation (n = 8).