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. 2016 Dec 13;11(12):e0166657. doi: 10.1371/journal.pone.0166657

Fig 2. Proteomic analysis of hemoglobin expression.

Fig 2

(A) Ratio of spectra (%) matched specifically to Hb tryptic peptides (spectral count) over total matched spectra during erythropoietic differentiation of cord blood-derived CD34+ cells along with human control RBCs and filtered cells on day 18. (B) Intensity based absolute quantification (iBAQ) was used to calculate the mole % ratio of peptides unique to α, β and γ Hb subunits over all identified peptides of filtered (F) and non-filtered (NF) cells on day 18 of culture along with human control RBCs. Other Hb subunits were found but were <0.5% of total Hb and are thus not shown on the graph. Standard deviations for α, β, γ, and δ are as follows: (NF): 3.4%, 2.8%, 3.0%, and 0.05%; (F): 4.2%, 9.7%, 5.8, and 0.23%; (control) 6.8%, 7.0%, 0.05%, and 0.36%, respectively.