Table 1. Summary of the stepwise filtering protocol applied to the hybridized microarrays.
Quality control step | Feature | Discarded features (%) | |
---|---|---|---|
Training set | Test set | ||
1: Spot filter | Individual spots | 0.20 | 4.05 (PR spots); 1.27 (RT spots) |
2: Probe filter 1 | Probes overlapping >25% * | 9.09 | 9.09 |
3: Probe filter 2 | Duplicated probes * | 10.13 | 10.13 |
4: Array filter | Full microarrays | 4.46 | 4.16 (PR region); 0 (RT region) |
* The discarded probes in the training set were not used in the test set, while no extra probes were discarded in the test set. As a result, the % of discarded probes is equal in both sets.