Fig 5. Phospholipase C activity testing.

Time-course hydrolysis of phosphatidylcholine (PC) with 100μg of wild type (blue circles) and His39Arg mutant (red circles) recombinant PlcA. The release of phosphocholine was measured indirectly by the fluorescence measurement of resorufin (AU) released using the Amplex red phosphatidylcholine kit, and continuously monitored at λ_exc of 510 nm and λ_em of 590 nm. Blue and red curves represent the mean of three independent experiments (coefficient of variation was 0.0195 and 0.0293, respectively).