Table 4. Number of contigs, genome coverage, and RT-PCR detections.
Label of the vine | Virus detected | contigs identified by BLAST | reads assembled by SOAP | coverage (reads mapped to reference) % | confirmation by RT-PCR |
---|---|---|---|---|---|
G1 | GLRaV-1 | 172 | 38 984 | 74 | positive |
GRSPaV | 113 | 3 208 | 46 | positive | |
GVA | 71 | 3 052 | 99 | positive | |
GVB | 83 | 16 856 | 99 | positive | |
GRVFV | 89 | 4 961 | 33 | positive | |
GSyV-1 | 2 | 1 086 | 68 | positive | |
HSVd | 5 | 1 132 | 90 | positive | |
GYSVd-1 | 1 | 805 | 91 | positive | |
G2 | GRSPaV | 169 | 3 776 | 97 | positive |
GPGV | 72 | 9 285 | 98 | positive | |
GFkV | 123 | 14 399 | 90 | positive | |
HSVd | 4 | 1 423 | 94 | positive | |
GYSVd-1 | 4 | 3 273 | 100 | positive | |
G3 | GRSPaV | 185 | 7 755 | 100 | positive |
GPGV | 81 | 14 812 | 98 | positive | |
GSyV-1 | 37 | 1 292 | 24 | positive | |
HSV-1 | 6 | 1 313 | 100 | positive | |
GYSVd-1 | 3 | 2 483 | 100 | positive | |
G4 | GLRaV-1 | 153 | 35 016 | 78 | positive |
GRSPaV | 71 | 1 222 | 25 | positive | |
GVA | 58 | 5 900 | 100 | positive | |
GPGV | 2 | 238 | 18 | positive | |
GRVFV | 120 | 4 580 | 29 | positive | |
HSVd | 6 | 1 297 | 100 | positive | |
GYSVd-1 | 6 | 2 800 | 100 | positive | |
G5 | GRSPaV | 68 | 10 237 | 100 | positive |
HSV | 199 | 1 395 | 90 | positive | |
GYSVd-1 | 0 | 95 | 100 | positive | |
G6 | GLRaV-1 | 162 | 30 117 | 74 | positive |
GRSPaV | 3 | 6 111 | 99 | positive | |
GPGV | 19 | 4 003 | 99 | positive | |
HSV | 7 | 1 355 | 100 | positive | |
G7 | GRSPaV | 35 | 4 288 | 94 | positive |
GPGV | 39 | 5 942 | 95 | positive | |
GFkV | 60 | 30 408 | 97 | positive | |
GRVFV | 23 | 9 376 | 22 | positive | |
HSVd | 9 | 1 384 | 100 | positive | |
GYSV-1 | 8 | 2 331 | 100 | positive | |
G8 | GRSPaV | 18 | 2 935 | 97 | positive |
GPGV | 24 | 4 918 | 98 | positive | |
GRVFV | 54 | 9 560 | 29 | positive | |
HSVd | 6 | 1 025 | 100 | positive | |
GYSVd-1 | 9 | 2 199 | 100 | positive |