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. 2016 Dec 13;11(12):e0168028. doi: 10.1371/journal.pone.0168028

Fig 5. PPD-H1 AS during temperature changes.

Fig 5

(a) WT plants assessed using HR RT-PCR primers spanning the N-terminus region. I1R –intron 1 retention. (b) PPD-H1 exon 6 undergoes AS to produce four different protein-coding isoforms: A, the fully spliced (FS) transcript; B, transcripts with only the Alt3’ss E6 (-6 nt); C, transcripts with only the Alt5’ss E6 (-45 nt); and D, transcripts with both Alt3’ss E6 (-6 nt) and Alt5’ss E6 (-45 nt). (c) WT plants assessed using HR RT-PCR primers spanning the region between exons 5 and 7. To simplify histograms, the PTC fraction is made up of multiple transcripts containing: I6R, I6R & Alt3’ss E6 (-6 nt), and Alt5’ss E6 (+5 nt). Error bars are SEM of three biological replicates. The levels of total transcripts at the different time-points are shown by the height of the histogram bars relative to the 20°C value (100%). The levels of AS variant transcripts are illustrated as a proportion of these totals. Relative expression values of abundant AS events are shown in tables below the histograms with significantly different AS/FS ratio compared to 20°C data shown with an asterisk (P < 0.01).