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. 2016 Dec 13;11(12):e0168028. doi: 10.1371/journal.pone.0168028

Table 1. Abundant AS events in barley core clock and clock-associated genes.

Gene AS events % total transcript Observation
LHY Various IR at the 5’UTR (IR 1, 2 and 3) Together, they form up to 26% total transcripts at 4°C; These add up to 12 uAUG (up to 31 aa uORF)
Alt 3' ss Intron 1 12% total transcripts at 4°C; Adds 1 uAUG (31 aa uORF)
Intron 4 retained (I4R) 40% total transcripts at 4°C Creates PTC on Exon 5
Intron 8 retained (I8R) 37% total transcripts at 4°C Altered C-terminus coding region
PRR37 Cryptic intron E1 (-102 nt) 20% total transcripts at 20°C Loss of uORF coding for 48 amino acids
Intron 1 retained 17% total transcripts at 20°C Adds uORF coding for 45 amino acids that overlaps functional ORF by 35 nt
alt 3' ss E6 (-6 nt) 33% total transcripts at 20°C Altered protein coding sequence
alt 5' ss E6 (- 45 nt) and alt 3' ss E6 (-6 nt) 24% total transcripts at 20°C Altered protein coding sequence
alt 5' ss E6 (- 45 nt) 76% total transcripts at 4°C Altered protein coding sequence
GI E2 skipping and Alt 3’ ss I2 (+12 nt) Up to 30% total transcripts at 20°C Removes 3 uAUG (up to 18 aa uORF)
Alt 3’ ss I2 (+12 nt) Up to 22% total transcripts at 20°C -
alt 5’ ss E2 (-8 nt) and alt 3’ ss I2 (+12 nt) Up to 11% total transcripts at 20°C; -
CO2 Alt 3’ ss (+68 nt) 35% total transcripts at 20°C Creates PTC