Table 2. Primers used in this study.
| Primer | Sequencea, b | Purpose |
|---|---|---|
| PAampG-F | 5’GGGATCCCAACGCGCACGCTTGCGCGAGGA 3’(BamHI) | Cloning of ampG of PAO1 |
| PAampG-R | 5’GAAGCTTTCAGTGCTGCTCGGCGTTCTGGT3’(HindIII) | |
| ECampG-F | 5’GGGATCCATGTCCAGTCAATATTTACG 3’(BamHI) | Cloning of ampG of E.coli 7 |
| ECampG-R | 5’GAAGCTTTTACGTCAGATGCGTTTTTCG 3’(HindIII) | |
| ABampG-F | 5’GGGATCCATGTTCCGACAGCAAAATCTTTA 3’(BamHI) | Cloning of ampG of A. baumannii 2089 |
| ABampG-R | 5’GGTCGACTTATACAGTTTTAGCATCTTTCCA 3(SalI) | |
| VCampG-F | 5’GGGATCCTAGGTACAAGTAGTTGGGGCCAGG 3’(BamHI) | Cloning of ampG of V. cholerae 03 |
| VCampG-R | 5’GGTCGACCAGTCCTATTTTAGTAGGTCATTTT 3’(HindIII) | |
| PAampG-G29R | 5’AACACCAGCATGGTCGGCAGgcgGGCGGCGAAGCCGAGCA 3’ | G29R |
| PAampG-G29C | 5’AACACCAGCATGGTCGGCAGgcaGGCGGCGAAGCCGAGCA3’ | G29C |
| PAampG-G29D | 5’AACACCAGCATGGTCGGCAGgtcGGCGGCGAAGCCGAGCA3’ | G29N |
| PAampG-G29A | 5’AACACCAGCATGGTCGGCAGggcGGCGGCGAAGCCGAGCA3’ | G29A |
| PAampG-G29V | 5’AACACCAGCATGGTCGGCAGgacGGCGGCGAAGCCGAGCA3’ | G29V |
| PAampG-29-R | 5’CTGCCGACCATGCTGGTGTTCAACACCCTGTCGGTGTGGC3’ | |
| PAampG-A129T | 5’TCGATCGCGATGTCCTGGGTggtGGAGGCGAACGCCACCA3’ | A129T |
| PAampG-A129S | 5’TCGATCGCGATGTCCTGGGTggaGGAGGCGAACGCCACCA3’ | A129S |
| PAampG-A129P | 5’TCGATCGCGATGTCCTGGGTgggGGAGGCGAACGCCACCA3’ | A129P |
| PAampG-A129G | 5’TCGATCGCGATGTCCTGGGTgccGGAGGCGAACGCCACCA3’ | A129G |
| PAampG-A129V | 5’TCGATCGCGATGTCCTGGGTgatGGAGGCGAACGCCACCA3’ | A129G |
| PAampG-A129D | 5’TCGATCGCGATGTCCTGGGTgtcGGAGGCGAACGCCACCA3’ | A129N |
| PAampG-129-R | 5’ACCCAGGACATCGCGATCGACGCCTACCGCCTGGAAATCG3’ | |
| PAampG-Q131E | 5’TAGGCGTCGATCGCGATGTCctcGGTGGCGGAGGCGAACG3’ | Q131E |
| PAampG-Q131P | 5’TAGGCGTCGATCGCGATGTCcggGGTGGCGGAGGCGAACG3’ | Q131P |
| PAampG-Q131R | 5’TAGGCGTCGATCGCGATGTCccgGGTGGCGGAGGCGAACG3’ | Q131R |
| PAampG-Q131H | 5’TAGGCGTCGATCGCGATGTCatgGGTGGCGGAGGCGAACG3’ | Q131H |
| PAampG-131-R | 5’GACATCGCGATCGACGCCTACCGCCTGGAAATCGCCGAGG3’ | |
| PAampG-A197T | 5’AGCCCAGGCAGGATCAGCAGggtGAACAACGCATAGGTCA3’ | A197T |
| PAampG-A197S | 5’AGCCCAGGCAGGATCAGCAGggaGAACAACGCATAGGTCA3’ | A197S |
| PAampG-A197G | 5’AGCCCAGGCAGGATCAGCAGgccGAACAACGCATAGGTCA3’ | A197G |
| PAampG-A197D | 5’AGCCCAGGCAGGATCAGCAGgtcGAACAACGCATAGGTCA3’ | A197N |
| PAampG-197-R | 5’CTGCTGATCCTGCCTGGGCTGGTCACCAGCCTGCTGATCC3’ | |
| PAampG-FqPCR | 5’GCGGTCTCGGTGATGGTGCT 3’ | qPCR |
| PAampG-RqPCR | 5’CGCTGGACGAACTCGGTGAT 3’ | |
| EC-FqPCR | 5’TGATGGACCGCTACACGC 3’ | qPCR |
| EC-RqPCR | 5’ACCCAGAACGCTGATTGC 3’ | |
| AB-FqPCR | 5’ACAGGCGCAACTCAAGAT 3’ | qPCR |
| AB-RqPCR | 5’CCCAATAAAGCAGCAACA 3’ | |
| VC-FqPCR | 5’TGACTGGCTGGCTGAAAG 3’ | qPCR |
| VC-RqPCR | 5’CGATGGATTGGCAGAAAA 3’ | |
| RpoD-FqPCR | 5’CCGTTGCTGAATATCCGGAA 3’ | qPCR |
| RpoD-RqPCR | 5’CAAATTTTTCGCGAGCCAGT 3’ |
a: The underlined sequences are restriction endonuclease sites.
b: The nucleotide sequence corresponding to the mutated amino acids are in lowercase.