. 2016 Dec 13;11(12):e0168060. doi: 10.1371/journal.pone.0168060

Table 4. Summary of ampicillin MIC, AmpC β-lactamase activity and qRT-PCR of ampG RNA results.

Strain	MIC(μg/ml)	β-Lactamase activity^a		qPCR(2^-ΔΔCt)
Strain	MIC(μg/ml)	Non-induced	Induced	qPCR(2^-ΔΔCt)
ATCC 25922	4	0	0	-
PAO1	512	112.40±7.2	1981.64±56	1.00±0.0
PAO1ΔampG	32	35.49±2.5	44.82±3.3	0.08±0.0
PAO1ΔampG-ampG_PA	1024	37.87±1.8	5019.31±98.2	8835.75±45.4
PAO1ΔampG-ampG_EC	512	118.50±7.0	1622.63±34	-
PAO1ΔampG-ampG_AB	1024	49.48±4.2	1680.35±28	-
PAO1ΔampG-ampG_VC	512	24.40±1.5	5063.64±67.3	-
PAO1ΔampG-UCP24-ampG_PA-A129T	64	309.64±10.6	139.37±6.6	10363.27±128.3
PAO1ΔampG-pUCP24-ampG_PA-A129S	1024	205.27±7.8	9556.45±132	6758.90±47.2
PAO1ΔampG-UCP24-ampG_PA-A129P	1024	895.45±66.8	12450.43±524	8803.84±56.9
PAO1ΔampG-pUCP24-ampG_PA-A129G	512	262.83±8.9	5342.68±88.7	9738.85±123.3
PAO1ΔampG-pUCP24-ampG_PA-A129V	64	200.74±14	232.28±26	0.52±0.0
PAO1ΔampG-pUCP24-ampG_PA-A129D	64	233.23±12.5	576.69±35	6218.63±45.6
PAO1ΔampG-pUCP24-ampG_PA-Q131E	1024	282.83±10	12559.25±213	6097.70±78.5
PAO1ΔampG-pUCP24-ampG_PA-Q131P	1024	101.28±5.8	12518.16±432.8	6433.10±104.6
PAO1ΔampG-pUCP24-ampG_PA-Q131R	1024	186.91±3	12393.96±541	9838.86±218.9
PAO1ΔampG-pUCP24-ampG_PA-Q131H	1024	187.31±2.1	11109.25±426	13156.64±321.2
PAO1ΔampG- pUCP24-ampG_PA-G29R	512	179.91±3.8	5142.63±132	4140.62±34.0
PAO1ΔampG- pUCP24-ampG_PA-G29C	512	117.52±4.6	4928.87±265.4	4054.96±62.6
PAO1ΔampG- pUCP24-ampG_PA-G29D	1024	223.10±1.6	4750.43±192	9054.04±102.7
PAO1ΔampG- pUCP24-ampG_PA-G29A	64	40.15±4	236.33±12.3	0.74±0.0
PAO1ΔampG- pUCP24-ampG_PA-G29V	64	17.30±1	209.16±36	0.32±0.0
PAO1ΔampG-pUCP24-ampG_PA-A197T	1024	163.03±21	3453.21±43.2	5123.37±36.9
PAO1ΔampG-pUCP24-ampG_PA-A197S	64	32.21±2.2	145.28±5.7	0.63±0.0
PAO1ΔampG-pUCP24-ampG_PA-A197G	1024	170.69±6	5023.37±246	7865.3±44.8
PAO1ΔampG-pUCP24-ampG_PA-A197D	128	46.28±7.8	406.78±11.2	0.12±0.0

^a: Nanomoles of nitrocefin hydrolyzed per minute per milligram of protein.

^_, not tested.