Fig. 3.
Real-time RT-PCR confirmation of microarray data from Klotho-treated MO3.13 cells. qRT-PCR confirmation of the regulated gene sets from microarray data of Klotho-treated MO3 cells. Ninety-one regulated genes from the microarray study were tested for validation by qRT-PCR. Seventy-nine percent of the upregulated (a) genes and 90 % of the downregulated (b) genes were consistent using both methods. All results are statistically significant at p<0.05. The fold change, p value, and Klotho treatment time for the regulated genes are listed in c