Table 1.
Methods to deplete GIV
| Name | Sequence | Species | Method of Introduction |
Vector | Citations | Target |
|---|---|---|---|---|---|---|
|
GIV
shRNA 3' UTR |
5'- CCGGGCTTTCATTA CC AGCTCTGAACTCGA GTTCAGAGCTGGTA ATGAAAGCTTTTTTG -3' |
Monkey (COS- 7), Human (HeLa, MDA- MB-231, Hs578T), |
Lentivirus | pLKO.1 | (Lopez-Sanchez et al, 2015b; Ma et al, 2015d) |
Human |
|
GIV
shRNA1 |
5'- GAAGGAGAGGCAAC TGGAT-3' |
Human (MDA- MB-231, MCF-7, HUVEC, BTSC, MCF10A,LN229, SH-SY5Y) Monkey(COS-7, Vero) Rat (PC- 12) |
Lentivirus | pLKO.1-puro, pSIREN-RetroQ, pcPURU6b |
(Bhandari et al, 2015; Enomoto et al, 2009; Gu et al, 2014; Jiang et al, 2008; Kitamura et al, 2008; Leyme et al, 2016; Leyme et al, 2015; Mao et al, 2012; Midde et al, 2015; Muramatsu et al, 2015; Natsume et al, 2012; Ohara et al, 2012) |
Rat, Human, Mouse |
|
GIV
shRNA2 |
5'- AAGAAGGCTTAGGC AGGAATT-3' |
Human (MDA- MB-231, MCF-7, VSMC) |
Lentivirus | pLKO.1-puro | (Leyme et al, 2016; Leyme et al, 2015; Miyake et al, 2011) |
Rat, Human, Mouse |
|
GIV
shRNA3 |
5'- GGAACAAACAAGAT TAGAA-3' |
Monkey (COS-7, Vero), Rat(PC- 12), Human(BTSC, MCF10A, SH- SY5Y) |
retroviral | pcPURU6b, pSIREN-RetroQ |
(Enomoto et al, 2009; Muramatsu et al, 2015; Natsume et al, 2012; Ohara et al, 2012) |
Rat, Human, Mouse |
|
GIV
shRNA4 |
5'- AGCTGGAACTTCTT CATGA-3' |
N/A | Retrovirus | pcPURU6b | (Enomoto et al, 2009) | |
|
GIV
shRNA5 |
5'- CCGGAGGCAAGAGT TGAGGAATTAACTC GAGTTAATTCCTCAA CTCTTGCCTTTTTTG -3' |
Human (DLD1) | Lentivirus | LvUCTP | (Zhang et al, 2014) | Human |
|
GIV
shRNA6 |
5'- GATCCCCGTCAATA ATGATGCCTCACTTC AAGAGAGTGAGGCA TCATTATTGACTTTT T-3' |
Human (U251) | Lipofectamin e |
pGCsi- H1/Neo/GFP |
(Ni et al, 2015) | Human |
|
GIV/GIR
DIN siRNA1 |
5'- AACCAGGTCATGCT CCAAATT-3' |
Monkey (Vero, COS-7), Human (THP-1, DLD1, HeLa, LX- 2, HUVEC, MDA-MB- 231,HREC, KYSE, MG-63), Rabbit (SMC), Canine (MDCK) |
Oligofectami ne |
N/A | (Beas et al, 2012; Dunkel et al, 2012; Enomoto et al, 2005; Garcia-Marcos et al, 2009; Garcia-Marcos et al, 2012; Ghosh et al, 2008; Hu et al, 2015; Ichimiya et al, 2015; Ito et al, 2013; Jiang et al, 2008; Kitamura et al, 2008; Lin et al, 2014; Lin et al, 2011; Lopez-Sanchez et al, 2014; Lopez-Sanchez et al, 2013; Ma et al, 2015a; Mao et al, 2012; Midde et al, 2015; Sasaki et al, 2015; Shibata et al, 2013) |
Human |
|
GIV/GIR
DIN siRNA2 |
5' GAGGCAGACAGUGU CAUUATT-3' |
Human (A549, H520,) |
Oligofectami ne |
N/A | (Pan et al, 2015) | Human |
|
GIV/GIR
DIN siRNA3 |
5'- GCAACAAGCUUACC UCAAUTT-3 |
Mouse (podocytes) |
PTD-DRBD | N/A | (Wang et al, 2015) | Human |
|
GIV/GIR
DIN siRNA4 |
5'- AAGAAGGCTTAGGC AGGAATT-3' |
Human (KYSE, PC-3, HeLa, MG-63), Canine (MDCK) |
Oligofectami ne |
N/A | (Hu et al, 2015; Shibata et al, 2013; Tomiyama et al, 2015) |
Human, Dog |
|
GIV/GIR
DIN siRNA5 |
5'- GGACCAACCUGGAU GAAUATT-3' |
Rabbit (Smooth Muscle Cell) |
Lipofectamin e RNAMAX |
N/A | (Miyachi et al, 2014; Miyachi et al, 2015) |
Rabbit |
|
GIV/GIR
DIN siRNA6 |
5'- GGCAGAACAUCCAC UAGCATT-3' |
Rabbit (Smooth Muscle Cell) |
Lipofectamin e RNAMAX |
N/A | (Miyachi et al, 2014; Miyachi et al, 2015) |
Rabbit |
|
GIV/GIR
DIN siRNA7 |
5'- CCAGAAUGUACCGA GAUGAUU-3' |
Human (DLD1) | Lipofectamin e |
N/A | (Zhang et al, 2014) | Human |
|
GIV/GIR
DIN siRNA8 |
5'- CUUCAUUAGUUCUG CGGGAUU-3' |
Human (DLD1) | Lipofectamin e |
N/A | (Zhang et al, 2014) | Human |
|
GIV/GIR
DIN siRNA9 |
5'- CAAGAGUUGAGGAA UUAAAUU-3' |
Human (DLD1) | Lipofectamin e |
N/A | (Zhang et al, 2014) | Human |
|
GIV/GIR
DIN siRNA1 0 |
5'- GGACCAACCUUGAU GAAUAUU-3' |
Human (DLD1) | Lipofectamin e |
N/A | (Zhang et al, 2014) | Human |
|
GIV/GIR
DIN siRNA1 1 |
5'- GAAGGAGAGGCAAC TGGAT-3' |
Human (U-87) | GenePorter | N/A | (Gu et al, 2014) | Human |