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. Author manuscript; available in PMC: 2017 Dec 7.
Published in final edited form as: Curr Protoc Chem Biol. 2016 Dec 7;8(4):265–298. doi: 10.1002/cpch.13

Table 4.

Primers for detection of GIV mRNA

Gene Name Primer Sequence NCBI Ref Seq(s) Amplicon
length		 PCR
efficiency
CCDC88A (Mouse)  Fwd: 5’-GTGATCTCTACTGCTGAAGG-3’
Rev: 5’-TGTTGCTCCCTAGACCTGCT-3’		 NC_000077.6 185 bp 96.6%
CCDC88A (Human)  Fwd: 5’- ATCTCAACTGCCGAAGGAACT-3’
Rev: 5’-TGTTGCTCCCTAGACCTGCT-3’		 NG_031944.1 182 bp 97.7%
GAPDH (Human) Fwd: 5’-TCAGTTGTAGGCAAGCTGCGACGT-3’
 Rev: 5’-AAGCCAGAGGCTGGTACCTAGAAC-3’		 NG_007073.2 185 bp 98.2%
GAPDH (Mouse) Fwd: 5’- CTGCAGCCTCGTCCCGTAGAC A - 3’
 Rev: 5’-TGCCGTGAGTGGAGTCATACTGGA- 3’’		 NC_000072.6 181 bp 97.1%