Table 1. Primers used for PCR amplification of the Brachionus calyciflorus mitochondrial genome.
| Primer | Sequence (5’-3’) | Gene/size |
|---|---|---|
| chromosome-I | ||
| LCCYTBF | GTACTYCCTWGAGRTCAADTGTC | Cytb-1 |
| LCCYTBR | CCTARTTYATTAGRAATAGAKCG | 473 |
| 1LCF1 | TGCAGAGAATTTTATTCCTGC | Cytb-12S |
| 1LCR1 | TGTATGATGGTGGATCATCC | 6478 |
| LC12SF | AAGAMAAGGWTTAGATAYCC | 12S |
| LC12SR | CADAGGTGWCGGGYGATTTGT | 367 |
| 1LCF2 | GCTTAATTCGGATTTGAAAG | 12S-Nad1 |
| 1LCR2 | ATGTGTCTCTGCTAAAGTAC | 1860 |
| LCND1F | TAGYGCAGWCTATTTDTTACGA | Nad1 |
| LCND1R | AATCYTGACWCTAGTYCAGACTC | 211 |
| 1LCF3 | GATCTTTTAGGATGTCTTCAC | Nad1-16S |
| 1LCR4 | GAGCTAGTTAGAATTTTCAC | 1943 |
| LC16SF | GTACYCTGADTGTGCTRAGGYAGC | 16S |
| LC16SR | CGGTTTRAADTCAGAYCATRTA | 411 |
| 1LCF5 | ATTACTACCTCGATGTTGG | 16S-Cox3 |
| 2LCR4 | GAAAAGTGGTGGTTATACAG | 7693 |
| LCCOX3F | ACARGATTCCWTGGTAYACAKGT | Cox3 |
| LCCOX3R | CAGAYAACAWCTACGARGTRTCA | 146 |
| 2LCF5 | GTAGGTACTATTTTTTTATTCTATG | Cox3- Cox1 |
| 2LCR7 | TCTCTTTGTTGTACGAGAAC | 7611 |
| LCCOX1F | ACCRGTTGAAYTGTGTWTCCTCC | Cox1 |
| LCCOX1R | GCTDCAAYATAAHAAGYATKATG | 761 |
| 2LCF1 | CTTCAGTACCTTTACTTTGAG | Cox1- Cytb2 |
| 1LCR5 | CGAGTTAAAGTAGGATTACC | 1217 |
| chromosome-II | ||
| LCND4F | CCTHTAGYTCATGWGGRGGMTCC | Nad4-Nad5 |
| LCND5R | GGAYTTGDATAACRCATVAGT | 1676 |
| 2LCF3.1 | CTTCAACTCTAGTTACAGCAGGC | Nad5- Nad3 |
| LCDoR | CATATATTGGTATAGGGGTTG | 1859 |
| 2LCF3.4 | CTTTAGAGTGACTATCTTGG | Nad3- Nad6 |
| 2LCR1.6 | GCAGATTGCACAAGTATAACCA | 5873 |
| 2LCF1-7 | ATGACAATCTTATTAAGCTT | Nad6-Nad4 |
| 2LCR1.1 | CAAACAACAGTAACATAAACTAAG | 1496 |
| 18S | ||
| LC18SF | ACCTGGTTGATCCTGCCAG | 18S |
| LC18SR | GATCCTTCTGCAGGTTCAC | 1800 |