Fig 4. FACS analyses of FAMIN expression in PBMCs and granulocytes.

PBMCs and granulocytes from human healthy donors were co-stained for different cell markers and FAMIN. Neutrophils, monocytes and DCs were shown to be FAMIN⁺. The events in the displayed graphs were first identified and gated by forward and side scatter parameters. (A) Gating strategy for FAMIN⁺ monocytes (CD14⁺, black) and CD14^- neutrophils (CD15⁺, purple and CD16⁺, orange). (B) Gating strategy for FAMIN⁺ myeloid DCs (CD11c⁺, brown) and plasmacytoid DCs (BDCA2⁺, green) was obtained from gating the CD3^-CD19^- population followed by gating of CD14^-HLADR⁺ cells. (C) Gating strategy for FAMIN⁺ T-cells (CD3⁺, purple) and B-cells (CD19⁺, orange). Numbers in the outlined areas indicate percent cells in each cell subtype. Data are representative of three independent experiments.