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. 2015 Jan 5;5:7617. doi: 10.1038/srep07617

Figure 5.

Figure 5

(A) Cellular location of WSPTs after 24 h incubation with A549 cells. Hoechst 33342 (excitation: 405 nm; collection: 425 to 475 nm), WSPTs (excitation: 488 nm; collection: 540 to 640 nm). (B) Co-location imaging of PT and Lyso Tracker Blue DND-22 (0.5 μmol/L) in A549 cells. (a) phase contrast image; (b) fluorescence image of Lyso Tracker Blue DND-22 (excitation: 405 nm; collection: 415 to 460 nm); (c) fluorescence image of PT (excitation: 488 nm; collection: 540 to 640 nm); (d) overlapped image of (b) and (c). (C) Dual-channel fluorescence images of PT-ADA-PPR in A549 cells. (a) phase contrast image; (b) fluorescence image of the back bone of PT-ADA-PPR (excitation: 488 nm; collection: 500 to 600 nm); (c) fluorescence image of PPR (excitation: 559 nm; collection: 655 to 755 nm); (d) overlapped image of (b) and (c). The false color of Hoechst 33342 (nuclei stain), Lyso Tracker Blue DND-22 (lysosome stain), WSPTs and PPR is blue, blue, orange and red, respectively. [WSPTs] = 20 μM (in RU). [Hoechst 33342] = 10 μg/mL.