Skip to main content
. 2016 Dec 13;11(12):e0168387. doi: 10.1371/journal.pone.0168387

Fig 3. Quantitative PCR and western blot analysis of the eIF5B-knockdown induced deactivation of MAPK signaling pathways.

Fig 3

(A) Quantitative PCR analysis of mRNA expression of selected genes in MAPK signaling pathways (*p<0.05; **p<0.01; ***p<0.001; n = 3); and (B) Total cell lysates immunoblotted with MEK1, Erk1/2, phospho-Erk1/2 (Thr202/Tyr204), MEK3, p38 and phospho-p38 (Thr180/Tyr182) antibodies.