Table 1.
Template Dilutions Workflow.
| Nominal copies/reaction | [Plasmid] (c/μL) | Plasmid vol (μL) | Diluent vol (μL) | Total vol (μL) |
|---|---|---|---|---|
| N/A | 1.00E + 08 | 200.00 | ||
| N/A | 1.00E + 07 | 20.0 | 180.0 | 200.0 |
| 50000000 | 2.50E + 06 | 100.0 | 300.0 | 400.0 |
| 5000000 | 2.50E + 05 | 40.0 | 360.0 | 400.0 |
| 500000 | 2.50E + 04 | 40.0 | 360.0 | 400.0 |
| 50000 | 2.50E + 03 | 40.0 | 360.0 | 400.0 |
| 5000 | 2.50E + 02 | 40.0 | 360.0 | 400.0 |
| 500 | 2.50E + 01 | 40.0 | 360.0 | 400.0 |
| 50 | 2.50E + 00 | 40.0 | 360.0 | 400.0 |
| 0 | 0.00E + 00 | 0.0 | 400.0 | 400.0 |
Each dilution was prepared volumetrically from a master stock of 1 × 108 copies/μL. The dilutions were stored in single use 50 μL aliquots at 20 °C for the duration of the study (1 month). For each dPCR and qPCR experiment, 20 μL was added to the 50 μL reaction.