Table 1.

Possible reaction configurations for duplex reactions.

		Number of primer pairs
		1	2
Probes binding	same region	Competing duplex reaction; e.g. rare mutant detection (Fig. 1B)	N/A
	different region	Non-competing (hybrid) duplex reaction (Fig. 1C)	Non-competing duplex reaction; e.g. copy number variation (Fig. 1A)