Table 2.

Experimental setup to assess an assay in duplex (with WT or variant templates).

Approximate template λ		Reaction format	Purpose
Variant	WT/other
0.7	–	Duplex	Evaluate SNV specificity (probe cross-hybridisation & filter bleed-through)
–	0.7	Duplex
0.7	–	Uniplex	Evaluate filter bleed-through
	0.7	Uniplex
0.7	0.7	Duplex	Evaluate partition specific competition and specificity
<0.05	–	Duplex	Evaluate SNV sensitivity and performance at low concentration (linearity)
<0.025	<0.025	Duplex	Evaluate probe cross-hybridisation & filter bleed-through