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Dualplex assay targeting TcdA and TcdB with Am5m3 and Bm5m3. The difference in quantification cycle (ΔCq) obtained at each concentration compared to the “no protein control” is plotted. PLAs were carried out in duplicate using two separate pools of 3′ oligonucleotide- and 5′ oligonucleotide-mAb targeting TcdA (Am5m3) and TcdB (Bm5m3). Error bars show standard deviations.
