Video 14. SCAR follows PIP₃ patches in macropinocytic cups of wild-type NC4 cells.

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DOI: 10.7554/eLife.20085.026

Detail of a vegetative cell from the wild strain NC4 expressing a marker for PIP₃ and a marker for the SCAR complex. Macropinocytosis is rapid and small but the cups still show SCAR puncta on their edges. Images were taken on a spinning disk microscope. It should be noted that due to the small size of the macropinocytic cups, the top and bottom of the cup are frequently in the focal plane, resulting in an overlap in both signals.

DOI: http://dx.doi.org/10.7554/eLife.20085.026