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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Oct 1;1863(1):21–32. doi: 10.1016/j.bbadis.2016.09.020

Fig 1.0. Parkin levels increase in lens epithelial cells exposed to H2O2-oxidative stress.

Fig 1.0

A. Immunoblot analysis of Parkin protein levels in 15 μg of total protein extract isolated from SRA 01/04 lens epithelial cells. SRA 01/04s were treated with indicated amounts of H2O2 in serum free media for 2 h and allowed to recover in complete media for 24 h. B. Immunoblot for GAPDH is shown as a control for equal protein loading. C. Densitometric analyses of the immunoblots plotted as Parkin levels as a ratio to GAPDH loading control.