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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Oct 1;1863(1):21–32. doi: 10.1016/j.bbadis.2016.09.020

Fig 4.0. Wt-Parkin recruits p62/SQSTM1 to damaged mitochondria in lens epithelial cells exposed to H2O2-oxidative stress.

Fig 4.0

SRA 01/04 lens epithelial cells (A-C) were transiently transfected with YFP-wt-Parkin and treated with either 200 μM H2O2 (B) or 10 μM CCCP (C) for 6 h and compared to untreated cells (A). Following treatment cells were immunostained for p62/SQSTM1 (pseudo green) and TOMM20 (pseudo red) and counterstained with DAPI (blue). Images were obtained using a confocal fluorescent microscope. All images were obtained using the 40 × objective. Cells overexpressing YFP-Parkin are outlined. In representative images, the overlay images show co-localization of p62/SQSTM1 (pseudo green) and TOMM20 (pseudo red) in YFP-wt-Parkin overexpressing cells (outline), resulting in yellow puncta. Examples of cells with yellow puncta are shown in large zoom for each treatment.