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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Oct 18;1863(1):200–210. doi: 10.1016/j.bbadis.2016.10.010

Figure 2.

Figure 2

Effects of TG enrichment on the chemical remodeling of plasma HDL under mild denaturing conditions. Native or TG-enriched HDL (nHDL and TG-HDL) that were either intact or have been incubated in 3M Gdn HCl for 3 h at 37°C were analyzed by (A) SEC (preparative-grade Superdex 16 XK column) and (B) native PAGE. SEC data of intact nHDL and of intact TG-HDL fully overlap and show a single peak centered circa 100 ml, which corresponds to intact-size HDL (not shown). Native PAGE demonstrates that, compared to nHDL, TG-HDL show increased dissociation of free protein and lipoprotein fusion upon Gdn-induced remodeling.