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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Oct 18;1863(1):200–210. doi: 10.1016/j.bbadis.2016.10.010

Figure 7.

Figure 7

Effect of TG enrichment on the enzymatic hydrolysis of plasma HDL. (A) HDL remodeling by LpL. LpL hydrolyses TG in the HDL core leading to apoA-I release accompanied by a decrease in the HDL size (see parts 2.2 and 3.3 for detail). Native PAGE shows that this process is more advanced in TG-HDL as compared to nHDL. (B) Proteolysis of apoA-I on HDL by MMP-12 (see parts 2.2 and 3.2 for detail) monitored by immunoblotting for apoA-I shows more advanced protein cleavage on TG-HDL as compared to nHDL. Numbers on the right correspond to full-length apoA-I (28 kDa), its N-terminal 26 kDa fragment cleaved near the flexible hinge (G185, G168), and its ~14 kDa fragments produced upon apoA-I cleavage in the flexible central region near residue 130 [57].