Table 4.

Comparison of gene expression profiles of α-TOH deficient neurodegenerative phenotypes. All mouse and rat assessments were performed using microarrays (Affymetrix Mu74Av2 and Affymetrix GeneChip Rat Expression Array 230A, respectively). Dietary vitE provided as all-rac-α-tocopheryl acetate to mice and rats. Transcripts are included if fold change > or < 2 (microarray) or pathways/modules were significant (RNA-seq)

Reference	(29)	(28)	(29)	(30)	Current study

Genotype	Ttpa^−/−	Ttpa^−/−	Ttpa^−/−	Wild-type Wistar rats	eNAD
Age	Birth	3–4 m	1 y	14 mo	1–2 y
Dietary vitE	35 IU/kg	35 IU/kg	35 IU/kg	<1.25 mg/kg vs 5 mg/kg	Variable
Tissue	Cerebral cortex	Cerebral cortex	Cerebral cortex	Cerebral cortex	Spinal cord

Downregulated: Synaptic vesicle trafficking	CPLX2	CPLX2		SYT5	RIMS1
	CPLX1	CPLX1		SLC17A7 (vGLUT1)	SYT1
		VAMP2			SYN2
		ARF3			UNC12
		SYT11			RIMS2
		SNCB			SYT7
					SYT11

Downregulated: Central myelination	PLP	PLP	PLP		MBP
Downregulated: Central myelination	MOBP	MOBP	MOBP

Downregulated: Nuclear receptors		RORA	RORA		NR4A2^*
Downregulated: Nuclear receptors					NR4A3^*

Downregulated: Ionotropic Glutamate receptors		GRIA1			GRIA1
		GRIN1			GRIK3
					GRIN1

Dysregulated: K⁺ Channels		KCNJ4 (downregulated)		KCNJ6	KCNJ4^*
				KCNJ11	KCTD12^*
				KCNH4 (downregulated)	KCNQ1^* (upregulated)

Downregulated: Antioxidant Enzymes (CAT, GPX1, SOD1)				CAT

Upregulated: LXR-target genes					CYP7A1^*
					APOE^*
					PLTP^*
					ABCA1

denotes significance at the single transcript level at an FDR<0.05 for eNAD