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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Immunobiology. 2016 Oct 5;222(2):188–197. doi: 10.1016/j.imbio.2016.10.003

FIG 3.

FIG 3

Pneumocystis induces arginase expression in vitro in a partially IL-4Rα-dependent manner. Primary alveolar macrophages from BALB/c or IL-4Rα−/− mice were cultured in quadruplicate in the presence of Pneumocystis (PC) organisms (MOI=10), IL-4/13+LPS, or media only and harvested after 24 hours. Cell lysates were collected and assayed for arginase activity as determined by measurement of the conversion of arginine into urea and standardized to total protein. Data is presented as fold increase compared to unstained controls for each cell type among CD11c+ cells. Means ± standard deviation are presented and are representative of 3 separate experiments. Statistically significant differences between treatment groups are denoted as follows: *, P < 0.05 and **, P < 0.01 as analyzed one-way ANOVA and Bonferroni post test. Asterisks atop of bars indicate comparison to media control condition, with differences between Pneumocystis and IL-4/13 treatments denoted by brackets.