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Immunoblot of Vasa and Vtg1 and 2. Three day old larvae were cut and fixed at time 0 (just after the cut), 6h or 24h after the cut. Samples were loaded on a gel for western blot. (A) Tubulin was used as a loading control. The protein bands were quantified using Image J (B,C,D) The protein expressions of vtg1, vtg2, and Vasa are not affected during regeneration.
