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. Author manuscript; available in PMC: 2016 Dec 13.
Published in final edited form as: Mech Dev. 2016 Aug 20;142:10–21. doi: 10.1016/j.mod.2016.08.003

Figure 4.

Figure 4

Transcriptional (A,B), and translational activity (C,D) in anterior and posterior pieces. A) Representative images of transcriptional activity in cut and uncut bipinnaria larvae, 3 days of age, B) Quantification of transcriptional activity. Sea star larvae were cut in half and incubated with EU (red), for either 6h or 24h before being fixed. The nuclei were labeled with Hoechst (blue). Uncut larvae were used as a reference. C) Representative images of translational activity in cut and uncut bipinnaria larvae, D) Quantification of translational activity. Sea star larvae were cut in half and fixed 6h and 24h later, after a 30 minute incubation with HPG (green) to visualize protein synthesis in situ. The nuclei were labeled with Hoechst (blue). Uncut larvae were used as a reference.