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. Author manuscript; available in PMC: 2016 Dec 13.
Published in final edited form as: Mech Dev. 2016 Aug 20;142:10–21. doi: 10.1016/j.mod.2016.08.003

Figure 5.

Figure 5

SRAP expression in normal development of P. miniata. To determine the temporal expression of the SRAP transcript in Pm, an RNA probe was synthesized for in situ hybridization and a probe against the neomycin-resistance gene was used as a negative control (A). The expression was tested in immature (a) and mature (b) oocytes, blastula (c), mid (d) and late gastrula (e), 4 day larva (f), and 6 day larva (g). The strongest expression was detected in the stomach of the larvae. qPCR was used to measure the RNA levels of SRAP at the indicated developmental stages: immature oocytes, hatched blastula, mid and late gastrula, 3 day, 4 day and 7 day old larvae (B). All values were normalized against the 18S mRNA and represented as a fold-change relative the amount of RNA present in hatched blastula.