Fig. 2. Phenotypic changes in microglia induced by diesel exhaust exposure.

Left: Microglia of DE-exposed mice showed morphological changes indicating a heightened state of reactivity. Area of microglial soma (µm²) increased, while circularity ($C=4\pi ·\frac{\mathit{\text{Area}}}{{(\mathit{\text{Perimeter}})}^2}$, a measure of inverse aspect ratio) was reduced. Results represent the mean (± SE) of six mice per group. Significant difference between FA and DE, ***p<0.001, *p<0.05. Right: Micrographs of Iba1 immunohistochemistry of adult mouse dentate gyrus following exposure to FA (top) or DE (bottom) are shown at 20× (left) or 40× (right).