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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2016 Sep 25;101:20–31. doi: 10.1016/j.freeradbiomed.2016.09.020

Figure 7.

Figure 7

Analysis of the reaction of NO with RSS·. (a) Amperometric detection of NO consumption. The trace shows the rate of decay of an NO-induced current in a pure NO purged flask containing 100 mM phosphate buffer (pH 7.4) containing 50 μM DTPA (0–15 min), after the addition of 2.5 mM TEMPOL (15–30 min) and after subsequent addition of 500 μM MCPD. (b) Amperometric measurements for the detection of NO resulting from reactions of GSNO with MCP-SSH or NAP. All measurements were performed anaerobically in 0.1 M phosphate buffer (pH 7.4) with 50 μM DTPA. The buffered solutions were allowed to equilibrate for 10 minutes prior to the addition of 100 μM GSNO. Dashed line: After NAP addition. (c) Computational evaluation of the reaction of a model alkyl perthiyl radical species (CH3-SS·) and NO.