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. 2016 Dec 12;213(13):2897–2911. doi: 10.1084/jem.20160167

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© 2016 Abboud et al.

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Figure 2. — CD27^hi memory CD8 T cells expand more than CD27^lo subset regardless of their tissue of origin. Mice that received spleen- (A and B) or lung-derived (C and D) CD8⁺CD44^hi CD27^hi and CD27^lo memory T cells were analyzed on day 7 after lethal infection. (top) Representative FACS plots showing total VACV-specific donor (CD45.1⁺) cells as a percentage of CD8⁺CD44^hi cells. (bottom) Representative FACS plots showing B8R_20-27/K^b tetramer⁺ cells from the host (CD45.1⁻) and each of the transferred (CD45.1⁺) CD27^hi and CD27^lo populations as indicated. Naive mice that did not receive any memory cells (NT) were used as controls. Numbers indicate percentages of positive cells within the gated population. Total lung (A and C) and spleen (B and D) CD45.1⁺ and B8R-reactive numbers presented as the mean ± SEM of three separate experiments with similar results containing three to four mice per group. *, P < 0.05; **, P < 0.01 (two-tailed Student’s t test).