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. 2016 Dec 12;213(13):2931–2947. doi: 10.1084/jem.20160303

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© 2016 Li et al.

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Figure 4. — Development and maturation of multiple immune cell subsets are compromised in Irf2^−/− mice at baseline and upon SVN infection. (A) Bulk splenocytes from uninfected and SVN-infected WT and Irf2^−/− mice on days 1, 2, and 5 p.i. were isolated and stained with antibodies specific for T cell (T), B cell (B), granulocyte (Gran), macrophage (Mac), DC, monocyte (Mono), and NK cell surface markers for flow cytometric analyses (see Fig. S1 for gating strategy). The graphs show frequencies of various immune cell populations as percentages of live splenocytes (black bars, WT; red bars, Irf2^−/−). (B–E) The percentages of CD4⁺ and CD8⁺ T cells (B), CD8⁺ and CD11b⁺ cDCs (C), NK subsets with increasing maturation indicated by color intensity (D), and Ly6C^hi and Ly6C^lo monocytes (E) are shown. Black open triangles and red closed circles represent WT and Irf2^−/− mice, respectively. Spleens from two to seven mice per genotype per time point were analyzed in a total of seven independent experiments. Error bars represent SD. Bonferroni posttests: *, P < 0.05; **, P < 0.01; ****, P < 0.0001.