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. 2016 Dec 12;213(13):2989–3005. doi: 10.1084/jem.20160817

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© 2016 Barrott et al.

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Figure 2. — Pten silencing promotes synovial sarcoma metastasis. (A) Gross photomicrographs of pulmonary lobes from hSS;Pten^w/w and hSS;Pten^fl/fl mice that were injected with TATCre at age 1 mo. (B) Graph depicts the fraction of mice in each group with metastasis detectable by gross inspection alone (hSS;Pten^w/w, n = 10, hSS;Pten^fl/fl, n = 88). (C) Low power H&E photomicrographs of lungs from mice bearing control or Pten-silenced tumors, as well as higher power H&E (right, from box in center image). (D) Graph depicts the fraction of mice in each group with metastasis detectable by histology. (E) Photomicrographs of lungs from tumor bearing hSS;Pten^fl/fl mice, demonstrating immunohistochemistry against GFP (indicates SS18-SSX expression). (F) Quantitative PCR results from lung tissue derived from tumor-bearing mice for the recombined Rosa26 locus as a marker of disseminated tumor cells. Two positive control samples that demonstrated metastasis (met.) on histology (one of each Pten genotype are noted in magenta), followed by testing for additional samples of each genotype without histologically detectable metastasis. Data points represent four technical replicates for each biological sample. (G) Graph depicts the estimated combined fraction of mice in each group with disseminated tumor cells detected by gross dissection, histology, or qPCR. Bars, 250 µm.