Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Dec 12;213(13):2989–3005. doi: 10.1084/jem.20160817

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Barrott et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 3. — Pten silencing drives increased vascularity in synovial sarcoma. (A) Plot of tumor mass at harvest against time elapsed after TATCre injection into mice bearing conditional SS18-SSX and either Pten^w/w (triangles) or Pten^fl/fl (circles). Filled shapes indicate tumors with associated histologically detected metastasis. (B) Pie chart demonstrating histological subtype fractions for metastatic tumors in hSS;Pten^fl/fl mice (n = 59). (C) Flow cytometry of a primary tumor and lung metastasis stained with E-cadherin and analyzed for intrinsic GFP expression. Tumor population (open arrow) and endothelial cells (filled arrow) both express higher levels of E-cadherin than the immune infiltrates (lower left). (D) Example photomicrographs of tumors with retained histological appearance between primary tumor and metastasis. (E) Example anti–Ki-67 immunohistochemistry photomicrographs and chart of proliferative indices for SS18-SSX-induced tumors in each Pten genotype (student’s t test). (F) Example H&E photomicrographs of necrosis (right side of each) identified in tumors and graph of the mean necrotic area per tumor cross section by histology (Student’s t test). (G) Example anti-CD31 immunohistochemistry photomicrographs demonstrating vascular density in hSS tumors arising with wild-type or silenced Pten. Graph presents mean vascular density in primary tumors of each genotype (Student’s t test). (H) Plot of vascular density indicated as vessel perimeter per cross-sectional area against cross-sectional area of each metastasis in hSS;Pten^fl/fl mice. Bars, 50 µm.