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. 2016 Dec 12;213(13):2949–2966. doi: 10.1084/jem.20160877

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© 2016 Hayano et al.

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Figure 1. — Expression of Netrin-4 in the adult rat and human spinal cord. (A) Spinal cord sections from heterozygous (Tp/+) rats showing LacZ⁺ cells (blue). (B and C) Higher magnification of the dorsal horn (B) and adjacent Nissl-stained sections (blue; C). DC, dorsal column. (D) Netrin-4 immunohistochemistry in the superficial dorsal horn. (E–I) Coimmunostaining with cell type–specific markers (red): NeuN (E), serine racemase (SR; F), GFAP (G), CD11b (H), and CD31 (I). Arrowheads indicate colocalization of LacZ and markers. (J) Netrin-4 immunohistochemistry in human lumbar cord sections from individuals with multiple sclerosis. (K) Higher magnification image of J. Bars: (A) 0.2 mm; (B, C, and E–I) 10 µm; (D) 0.1 mm; (J and K) 0.5 mm. Each experiment was performed twice.