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. 2016 Dec 12;213(13):3075–3086. doi: 10.1084/jem.20160888

Figure 2.

Figure 2.

Local cognate antigen expression dramatically amplifies generation of antiviral skin TRM cells. (A) Mice were adoptively transferred with 5 × 104 OVA-specific CD45.1+ OT-I cells 1 d before infection (i.v.) with MVA-OVA. Immediately after i.v. infection, mice were divided into three groups. Group 1 was mock infected (PBS mock) on the right ear (R). Group 2 was mock infected (PBS mock) on the left ear (L) and infected with MVA-WT on the right ear (R). Group 3 was infected on the left (L) and right ear (R) with MVA-WT and MVA-OVA, respectively. 7 d (Acute) and 40 d (Memory) pi, total numbers of OT-I cells were determined in differentially inoculated ears. (B and C) Representative plots depicting the frequency of OT-I cells (among CD45+ cells) in the indicated skin region 7 d (B) or 40 d (C) pi (top). Total number of OT-I cells in each ear 7 d (B) and 40 d (C) after inoculation (bottom). Data are derived from one experiment representative of at least two independent experiments (Acute, n = 4; Memory, n = 5). Dots in graphs show individual mice and bars show mean + SEM. *, P < 0.05; **, P < 0.01 (paired Student’s t test).