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. 2016 Dec 12;213(13):2913–2929. doi: 10.1084/jem.20161289

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© 2016 Khan et al.

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Figure 4. — Vaginal LCMV infection delays priming of virus-specific CD8 T cells. (A–F) P14 immune chimeras were generated by adoptively transferring 500,000 CFSE-labeled CD45.1⁺ P14 cells into naive CD45.2⁺ B57BL/6N mice. (A) CD69 and CD25 expression patterns and CFSE dilutions of P14s in the iLN 3 d after infection. (B) CD44 expression on P14s from A. MedFI, median fluorescence intensity. (C and D) CD69 and CD25 expressions on P14 cells in the indicated tissues at day 3 (C) or the indicated days (D) after i.p. or i.vag. infection. (D) CFSE dilution of P14 cells is shown on the right. (E) Frequency of CFSE^lo P14 cells from D. UI, uninfected. (F) IFN-γ and IL-6 mRNA levels in iLN samples from D were measured by qRT-PCR, and their levels were normalized to GAPDH and expressed as fold-change over uninfected controls. Data are represented from one of five (D and E), three (A–C), or two (F) independent experiments. n = 3 (A–C; days 0–4 in E and F) or 4 (day 5 in E). Error bars represent mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001, unpaired Student’s t test.