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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Oct 19;1863(1):68–80. doi: 10.1016/j.bbadis.2016.10.017

Figure 1. Structure of the dPrx5 transgenic constructs.

Figure 1

The UAS-dPrx5 construct coding for wild type full length dPrx5 polypeptide was made previously (14). Briefly, the construct included a mitochondrial pre-sequence (black), which is subsequently cleaved at a second in-frame methionine upon entry into the mitochondria. This second in-frame methionine also serves as an alternative translation site giving rise to the short dPrx5 form (in white), found in cytosol and nucleus (14). The SOD2 mitochondrial leader peptide (in dark grey) and nuclear-targeting SV40 NLS sequence (in dark grey) were used to target dPrx5 to the mitochondrial and nuclear compartments respectively while removal of the mitochondrial targeting domain restricted expression of dPrx5 largely to the cytosolic compartment, and secondarily to the nucleus.