The genotypes of flies subjected to analysis were Da-GAL4, dprx5/+ (control); Da-GAL4, dprx5/dprx5 (dprx5 mutant); Da-GAL4, dprx5/RNAidprx3 (dprx3) and Da-GAL4, dprx5/RNAi-dprx3, dprx5 (DM). A, Survivorship of Prx single and double mutants along with control. RNA was extracted for RNA-seq analysis before the onset of the rapid death observed in the DM at 13 day, indicated by the arrow. B, Venn diagram representing the overlapping and non-overlapping genes, whose expression was altered in the double and single mutants compared to control. The diagram shows the total number of up-regulated and downregulated genes unique for each group. G1, G2, G3 and G4 – groups of overlapping up- and downregulated genes, shown in the table. C, Functional clustering of 131, 70 and 81 genes up-regulated in dprx3, dprx5 and double mutants respectively. D, Functional clustering of 175, 86 and 94 genes down-regulated in dprx3, dprx5 and double mutants respectively. Biological processes GO with an enrichment score >1 are depicted. Suppl. Table 1 contains lists of the genes included in B–D.