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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Oct 19;1863(1):68–80. doi: 10.1016/j.bbadis.2016.10.017

Figure 7. Immunoblot analysis of subcellular fractions isolated from fly lines expressing different dPrx5 forms.

Figure 7

dPrx5 protein expression from different UAS transgenes was assessed in the dprx5 null background. Genotypes of fly lines were as follows: cytosolic – UAS-cyt dPrx5/+; Da-GAL4, dprx5/dprx5; nuclear – UAS-nucl dPrx5/+; Da-GAL4, dprx5/dprx5; mitochondrial – UAS-mit dPrx5/+; Da-GAL4, dprx5/dprx5. For each form, three independent fly lines were generated and immunoblot analysis was performed in triplicate. Flies expressing the endogenous dPrx5 protein served as a positive control (C). Antibodies raised to compartment-specific proteins (GCLm, Histone H3 and dPrx3) were used to control for loading and purity of subcellular fractions.