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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Gynecol Oncol. 2016 Mar 11;141(2):348–356. doi: 10.1016/j.ygyno.2016.02.030

Figure 4. NR4A1 in human serous ovarian cancer cell lines.

Figure 4

Figure 4A. Immunoblot for NR4A1 in whole cell lysate of several cell lines representing HGS OVCA. Figure 4B. Densitometry analysis on immunoblot in A showing OVCAR-432 and OVCAR3 have significantly higher NR4A1 expression. Figure 4C. Representative immunofluorescent images of indicated cell lines stained for NR4A1 and Hoechst. Images are representative of 20x magnification with a digital zoom inset. Figure 4D. Analysis of total NR4A1 immunofluorescence signal in representative cell lines. Image analyses are representative of: PEO1(5 experiments), OVCA432(2 experiments), OVCAR8(3 experiments), OVSAHO(2 experiments), CA-OV-3(2 experiments), and OVCAR3(2 experiments). NR4A1 data from D was analyzed for nuclear/cytoplasmic localization. The fraction of NR4A1 in the cytoplasm was calculated by normalizing the integrated fluorescence intensity in the cytoplasm to the total integrated intensity in the cell (fluorescence in the cytoplasm and nucleus).