Table 1. Characteristics of CLL clonotypes and mimotopes.
| Patient | Clonotype | mIgCLLa | VHDJH | VLJL | CDR3 lengthb | VH CDR3 IMGT aa sequencec | Freq. (%)d | Stereotypic subsete | Mimotope name | Mimotope aa sequence | KD (nm)f |
|---|---|---|---|---|---|---|---|---|---|---|---|
| CLL#1 | CLL1 | mIgCLL1 | V3-11/D3-10/J6 | κV1-39(1D-39)/J2 | 17 | AREGLWFGELSYYGMDV | 100 | NA | pCLL1 | CSPAKELGC | 34.03 |
| CLL#2 | CLL2 | mIgCLL2-1 | V3-73/D3-3/J6 | κV1-33(1D-33)/J1 | 20 | TFDFWSGYYDGYYYYYGMDV | 80 | NA | pCLL2-1 | CNTYSVSLC | 10.81 |
| mIgCLL2-2 | TFDFWSGYYDGYYYYYGLDF | 20 | pCLL2-2 | CKSYSVSLC | 7.57 | ||||||
| CLL#3 | CLL3 | mIgCLL3-1 | V3-23/D3-22/J4 | κV1-8(1D-8)J3 | 17 | AKRDYSHRSDYAPLFEY | 90 | NA | pCLL3-1 | CPPQSVTEC | 22.14 |
| mIgCLL3-2 | GKRDYSHSSDYAPLFEY | 10 | NA | pCLL3-2 | CDVWHSAYC | 5.64 | |||||
| CLL#4-1 | CLL4-1 | mIgCLL4-1 | V4-34/D7-27/J2 | κV1-33(1D-33)/J1 | 17 | ARRGTGDPPYWYFDL | 77 | NA | pCLL4-1 | CTTNPADSC | 5.50 |
| CLL#4-2 | CLL4-2 | mIgCLL4-2 | V4-4/D6-19/J2 | κV2-28(2D-28)J1 | 21 | ARGTVGQQWLEVLDWYFGL | 23 | NA | pCLL4-2 | CVLWWSPIC | 3.90 |
| CLL#5-1 | CLL5-1 | mIgCLL5-1 | V4-34/D3-22/J4 | κV1D-12/J1 | 20 | ARGGNNDKIVMLLYYFDF | 57 | NA | pCLL5-1 | CFSDDEWWC | 7.08 |
| CLL#5-2 | CLL5-2 | mIgCLL5-2 | V4-59/D3-22/J3 | κV1-13(1D-13)/J2 | 18 | ARDYDYDTRKSDAFDIW | 43 | NA | pCLL5-2 | CPPFTNYEC | 7.32 |
| CLL#6-1 | CLL6-1 | mIgCLL6-1 | V1-46/D3-10/J6 | κV3D-15/J1 | 22 | ARDWVATMVRGVIESRPTGMDV | 65 | NA | pCLL6-1 | CNQDWHKKC | 57.53 |
| CLL#6-2 | CLL6-2 | mIgCLL6-2 | V4-34/D2-2/J6 | κV1D-17/J1 | 24 | ASRFYCSGSSCEAPRYYYYYGLDV | 35 | 16 | pCLL6-2 | CTTVIPERC | 22.67 |
a
Name of the CLL-derived monoclonal Igγ1 antibodies expressing the indicated VHDJH and VLJL rearrangements.
b
Number of amino acids of the VH CDR3.
c
VH CDR3 amino acid sequence according to the International ImMunoGeneTics information system (http://www.imgt.org).
d
Percentage of analyzed cells expressing the indicated VH CDR3.
e
Stereotypic subset according to Agathangelidis et al.5 NA, not attributable to a currently defined stereotypic subset.
f
KD values for the mimotope binding to the cognate mIgCLL, as estimated by the Scatchard plot analysis.