Table 1.
Primary and secondary antibodies.
| Antibody | Source | Isotype | Clone | Application | Dilution |
|---|---|---|---|---|---|
| Connexin 43 | Santa Cruz Lab | Rabbit IgG | H-150 | WB | 1/1000 |
| Cytokeratin 12 | Abcam | Rabbit IgG | EPR1609(2) | WB | 1/2000 |
| Cytokeratin 12 | Santa Cruz Lab | Goat IgG | L-20 | IFI | 1/100 |
| Cytokeratin 19 | Boehringer Mannheim | Mouse IgG1 | 170.2.14 | WB, IFI | 1/1000, 1/50 |
| Cytokeratin 3 | Millipore | Mouse IgG1 | AE5 | WB, IFI | 1/2500, 1/100 |
| E-cadherin | BD Transduction Laboratories | Mouse IgG2a | 36 | WB | 1/2000 |
| p63 | Millipore | Mouse IgG2a | 4A4 | WB | 1/1250 |
| p63 | Abcam | Mouse IgG2a | BC4A4 | IFI | 1/50 |
| Tubulin | Sigma | Mouse IgG1 | DM1A | WB | 1/2000 |
| Phalloidin-TRITC | Sigma | IFI | 1/2000 | ||
| Goat anti-rabbit | Sigma | Goat | WB | 1/2500 | |
| Rabbit anti-mouse | Sigma | Rabbit | WB | 1/2500 | |
| Goat A488 | Invitrogen Life Technologies | Goat | IFI | 1/1000 | |
| Mouse A488 | Invitrogen Life Technologies | Mouse | IFI | 1/1000 |
WB: Western blot; IFI: indirect immunofluorescence.