Figure 2.
Morphological changes in HeLa cells treated with R. officinalis (REO), C. longa (CEO), and Z. officinale (GEO) essential oils and dyed with Giemsa. (A) Positive control: cell population treated with DMSO; (B) negative control: cell population without influence of treatment; (C–E) cells treated with 31.12; 249; and 1992 μg/mL of REO; (F–H) cells treated with 32.81; 262; and 2100 μg/mL of CEO; (I–K) cells treated with 30.12; 241; and 1928 μg/mL of GEO. Cell density 1 × 106 cells/mL. Images taken at 40x magnification. (→ cell membrane protrusions (“blebs”); ▸ chromatin condensation; ⟶ cell content leakage and formation of apoptotic bodies.)